The STACKS setting determines the number(0,8-64) and size(32-512) of the stack spaces that are created to handle interrupt handling in real mode (Note if set too low it can cause stack overflow in Windows) - Default Value 9,256.
The LASTDRIVE setting specifies how many driveletters(A-Z) available to a DOS programn - Default your last drive letter.STACKSHIGH=9,256
The FILES setting sets the number of File Handles(8-255) available to a DOS program to have open - Default value 30.LASTDRIVEHIGH=Z
The BUFFERS sets the number of disk buffers(1-99) available for limited buffering of file input/output (I/O) that is handled by MS-DOS real-mode drivers - Default value 30.FILESHIGH=40
The FCBS (File Control BlockS) setting specifies the number of FCBS(1-255) available to a DOS program, but most DOS programs uses filehandles - Default value 4,0.BUFFERSHIGH=40
More Info MS KB82318FCBSHIGH=1,0
More Info MS KB145799
More Info MS KB274646
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